7.2 POJA-L1730+1728+1729
Title: Cervix, keratin profile (ectocervix, human, adult)
Description: Stain: antikeratin antibody immunoperoxidase staining with diaminobenzidin reaction (DAB) and hematoxylin counterstaining.
(A): Cytokeratin 4 expression (antibody 6B10) in non-keratinised stratified squamous epithelium (NKSSE) of the ectocervix. Generally superficial (1) and intermediate (2) cell layers express keratin 4 whereas parabasal (3) and basal (4) cells remain negative. (5) negative proper lamina.
(B): Cytokeratin 19 expression (antibody LP2K) in NKSSE. Only basal (4) cells exclusively express keratin 19. All other epithelial cells remain negative. (5) negative proper lamina.
(C): Cytokeratin 18 (antibody RCK 106) in NKSSE. Basal (4) and parabasal (3) cells specifically express keratin 18 while all other epithelial cells remain negative. (5) negative proper lamina. (Cytokeratin 4 and 19 obtained by courtesy of F. Smedts MD, PhD, former pathologist at the Department of Pathology, University Medical Center, St. Radboud University, Nijmegen, The Netherlands).
Background: Moving from endometrial epithelium to endocervical, ectocervical and vaginal epithelium the expression of the keratine profile changes dramatically concurrent with the type of epithelial cells, e.g. the change from simple epithelium to squamous epithelium and keratinized squamous epithelium. Those profiles are used for differential diagnosis of type of tumors since usually tumors retain the original keratin expression profile. Keratin 7 has been shown to distinguish between positive adenocarcinoma of the cervix and the negative adenocarcinoma of the colon, using antibody OV-TL12/30. The fact that keratin 7 was also detected in cervical reserve cells as well as in a considerable number of CIN lesions and cervical carcinomas has been said to suggest that reserve cells are a common progenitor cell for these lesions.
Keywords/Mesh: female reproductive organs, uterus, cervix uteri, keratin profile, histology, POJA collection.
Title: Cervix, keratin profile (ectocervix, human, adult)
Description: Stain: antikeratin antibody immunoperoxidase staining with diaminobenzidin reaction (DAB) and hematoxylin counterstaining.
(A): Cytokeratin 4 expression (antibody 6B10) in non-keratinised stratified squamous epithelium (NKSSE) of the ectocervix. Generally superficial (1) and intermediate (2) cell layers express keratin 4 whereas parabasal (3) and basal (4) cells remain negative. (5) negative proper lamina.
(B): Cytokeratin 19 expression (antibody LP2K) in NKSSE. Only basal (4) cells exclusively express keratin 19. All other epithelial cells remain negative. (5) negative proper lamina.
(C): Cytokeratin 18 (antibody RCK 106) in NKSSE. Basal (4) and parabasal (3) cells specifically express keratin 18 while all other epithelial cells remain negative. (5) negative proper lamina. (Cytokeratin 4 and 19 obtained by courtesy of F. Smedts MD, PhD, former pathologist at the Department of Pathology, University Medical Center, St. Radboud University, Nijmegen, The Netherlands).
Background: Moving from endometrial epithelium to endocervical, ectocervical and vaginal epithelium the expression of the keratine profile changes dramatically concurrent with the type of epithelial cells, e.g. the change from simple epithelium to squamous epithelium and keratinized squamous epithelium. Those profiles are used for differential diagnosis of type of tumors since usually tumors retain the original keratin expression profile. Keratin 7 has been shown to distinguish between positive adenocarcinoma of the cervix and the negative adenocarcinoma of the colon, using antibody OV-TL12/30. The fact that keratin 7 was also detected in cervical reserve cells as well as in a considerable number of CIN lesions and cervical carcinomas has been said to suggest that reserve cells are a common progenitor cell for these lesions.
Keywords/Mesh: female reproductive organs, uterus, cervix uteri, keratin profile, histology, POJA collection.