10.2 POJA-L2059+2060+
2063+2064.
Immunofluorescence keratin pattern in the epidermis of the thin skin
10.2 POJA-L2059+2060+2063+2064
Title: Immunofluorescence keratin pattern in the epidermis of the thin skin
Description:
(A): Back skin, stain hematoxylin-eosin, frozen section, human.
(B-E): Back skin, FITC-immunofluorescence staining with monoclonal antibodies against cytokeratin filaments, frozen section, human:
(B) RGE53, cytokeratin 18.
(C) RCK102, cytokeratin 5 and 8.
(D) OVTL12/30, cytokeratin 7.
(E) OVTL12/5, cytokeratin 5, 7, 14 and 19.
Cytokeratin 18 and 7 are not present in the epidermis (B, D), cytokeratin 5+8 are expressed in the stratum germinativum only (C),
while OVTL12/5 detects all epidermal epithelial cells.
Remarks: Soft keratins are divided into the type I (basic or neutral keratin 1 to 8) and type II (acidic keratins 9 to 20) subgroups.
RCK102 and OV-TL12/5 demonstrate the presence of cytokeratin 5, 8, 14 and 19 in the basal cell layer as well as partly
in the spinous cell layer.
Cytokeratin 5, 8, 14 and 19 are present in the basal layer and spinous (prickle) cell layers but the mature spinous cells towards
the granular layer lose the reactivity for the basic cytokeratin 5 and 8 (type I).
Upon maturing of keratinocytes towards the granular layer the acidic cytokeratin 14 and 19 (type II) remain expressed.
Corneocytes (squames) do not react with these antibodies.
Background: RGE53 is monospecific for keratin 18 and recognizes columnar epithelial cells and mesothelial cells, squamous
epithelium generally remains negative.
RCK 102 is a broadly reacting keratin antibody which recognizes cytokeratin 5 and 8.
OV-TL12/30 only recognizes keratin 7 generally in columnar epithelium (exception a.o. colon, prostate) cells and mesothelium.
OVTL12/5 is also a broadly reacting antibody which recognizes keratins 5, 7, 14, 19.
Keywords/Mesh: skin, epidermis, cytokeratin, histology, POJA collection
Title: Immunofluorescence keratin pattern in the epidermis of the thin skin
Description:
(A): Back skin, stain hematoxylin-eosin, frozen section, human.
(B-E): Back skin, FITC-immunofluorescence staining with monoclonal antibodies against cytokeratin filaments, frozen section, human:
(B) RGE53, cytokeratin 18.
(C) RCK102, cytokeratin 5 and 8.
(D) OVTL12/30, cytokeratin 7.
(E) OVTL12/5, cytokeratin 5, 7, 14 and 19.
Cytokeratin 18 and 7 are not present in the epidermis (B, D), cytokeratin 5+8 are expressed in the stratum germinativum only (C),
while OVTL12/5 detects all epidermal epithelial cells.
Remarks: Soft keratins are divided into the type I (basic or neutral keratin 1 to 8) and type II (acidic keratins 9 to 20) subgroups.
RCK102 and OV-TL12/5 demonstrate the presence of cytokeratin 5, 8, 14 and 19 in the basal cell layer as well as partly
in the spinous cell layer.
Cytokeratin 5, 8, 14 and 19 are present in the basal layer and spinous (prickle) cell layers but the mature spinous cells towards
the granular layer lose the reactivity for the basic cytokeratin 5 and 8 (type I).
Upon maturing of keratinocytes towards the granular layer the acidic cytokeratin 14 and 19 (type II) remain expressed.
Corneocytes (squames) do not react with these antibodies.
Background: RGE53 is monospecific for keratin 18 and recognizes columnar epithelial cells and mesothelial cells, squamous
epithelium generally remains negative.
RCK 102 is a broadly reacting keratin antibody which recognizes cytokeratin 5 and 8.
OV-TL12/30 only recognizes keratin 7 generally in columnar epithelium (exception a.o. colon, prostate) cells and mesothelium.
OVTL12/5 is also a broadly reacting antibody which recognizes keratins 5, 7, 14, 19.
Keywords/Mesh: skin, epidermis, cytokeratin, histology, POJA collection