5.7 POJA-L5024+5025+
5026+5027.
Urinary bladder wall and lining in sheep
5.7 POJA-L5024+5025+5026+5027
Title: Urinary bladder wall and lining in sheep
Description:
Bladder wall. (A): Stain hematoxylin-eosin and (B, C): Immunoperoxidase staining with DAB and antibodies against cytokeratin, desmin, vimentin and smooth muscle cell actin as indicated on the photographs.
(A): The sheep bladder consists of a mucosa with an urothelium anchored to the lamina propria layer of elastic connective tissue.
The submucosal layer contains an incomplete muscular layer (muscularis mucosae), followed by a well-developed detrusor muscle
bundle or tunica muscularis, consisting of several layers of muscle bundles forming a dense wickerwork, needed to empty the stored
urine. The detrusor muscle is under control of the sympathetic and parasympathetic nerves.
The sphincter muscle is not shown here.
(B): Both the urothelium and the perineurium are positively stained with antibodies against cytokeratin.
The perineurial cells are epitheloid myofibroblasts and contain also tight junctions, gap junctions, external laminae and are contractile.
The muscle cells are here positive for the desmin marker, confirming the incompleteness of the muscularis mucosae.
(C): Vimentin is found largely in the cellular elements of connective tissue in the lamina propria and in between the muscle fibers.
Actin marks both layers of smooth muscle cells.
(A - C, by courtesy of D. Tiemessen, BSc, Department of Urology, Radboud university medical center, Nijmegen, The Netherlands).
Keywords/Mesh: urinary system, urinary bladder, vesica urinaria, detrusor muscle, cytokeratin, vimentin, actin, desmin, histology, POJA collection
Title: Urinary bladder wall and lining in sheep
Description:
Bladder wall. (A): Stain hematoxylin-eosin and (B, C): Immunoperoxidase staining with DAB and antibodies against cytokeratin, desmin, vimentin and smooth muscle cell actin as indicated on the photographs.
(A): The sheep bladder consists of a mucosa with an urothelium anchored to the lamina propria layer of elastic connective tissue.
The submucosal layer contains an incomplete muscular layer (muscularis mucosae), followed by a well-developed detrusor muscle
bundle or tunica muscularis, consisting of several layers of muscle bundles forming a dense wickerwork, needed to empty the stored
urine. The detrusor muscle is under control of the sympathetic and parasympathetic nerves.
The sphincter muscle is not shown here.
(B): Both the urothelium and the perineurium are positively stained with antibodies against cytokeratin.
The perineurial cells are epitheloid myofibroblasts and contain also tight junctions, gap junctions, external laminae and are contractile.
The muscle cells are here positive for the desmin marker, confirming the incompleteness of the muscularis mucosae.
(C): Vimentin is found largely in the cellular elements of connective tissue in the lamina propria and in between the muscle fibers.
Actin marks both layers of smooth muscle cells.
(A - C, by courtesy of D. Tiemessen, BSc, Department of Urology, Radboud university medical center, Nijmegen, The Netherlands).
Keywords/Mesh: urinary system, urinary bladder, vesica urinaria, detrusor muscle, cytokeratin, vimentin, actin, desmin, histology, POJA collection