7.1 POJA-L1878+1879+1908+1909+1910+1911
Title: Papillary serous cystadenocarcinoma of ovary (human, adult)
Description: Case nr. p577. Stain: (A1) May-Grünwald-Giemsa (MGG); (A2) Papanicolaou (Pap); immunoelectron microscopy using peroxidase with diaminobenzidin reaction (DAB) (B1-2) OC125 antibody and (C1-2) OVTL3 anti-ovarian carcinoma antibody.
(A1): Cytospin preparation of malignant ascites with islets of reddish-stained tumor cells (1), bluish-stained larger mesothelial cells (2) and some of them contain vacuoles.
(A2): Ascites (Pap) with clustered small dense-stained tumor cells (1) and lighter-stained mesothelial cells with vacuoles ( ).
(B1): Ascitic tumor cell with large nucleus (3) and nucleolus with swollen mitochondria (4). Cell membrane and microvilli of tumor cell are coated with a thin electron-dense layer of DAB reaction product of OC125 antibody (→).
(B2): Higher magnification reveals numerous microvilli stained with OC125 antibody (→).
(C1): A cluster of three ascitic tumor cells shows membrane-bound OVTL3 reactivity (→) between the cells as well as at the free surface of top cell. Nuclei (3) and swollen mitochondria.
(C2): Higher magnification of electron-dense reaction product on epitopes (→) in membrane and microvilli. Cytoplasm with rough endoplasmic reticulum, mitochondria (4) and part of nucleus (3) remains unstained.
Background: See also Background POJA-L1476+L1760+L1770+L1750. The OC125 (MUC 16) antibody recognizes an epitope on a molecule called Cancer Antigen 125 (CA125) a.o. in tumor cell membranes. It is directed against a glycoprotein produced by human epithelial ovarian cancer cells. The antibody OVTL3 was generated against ovarian carcinoma cells and detect for the greater part cell membrane-bound epitopes of the carcinoma cells in the primary tumor as well as in distant metastases. The antibody was also applied for immunoscintigraphy. However cytoplasmic epitopes might be lost in suspended tumor cells (e.g. ascites).
Keywords/Mesh: female reproductive organs, ovary, OC125 antibody, OVTL3 antibody, ascites, histology, pathology, electron microscopy, female genitalia, ovarian neoplasms, papillary serous cystadenocarcinoma, ovarian tumor antigens, histology, POJA collection
Title: Papillary serous cystadenocarcinoma of ovary (human, adult)
Description: Case nr. p577. Stain: (A1) May-Grünwald-Giemsa (MGG); (A2) Papanicolaou (Pap); immunoelectron microscopy using peroxidase with diaminobenzidin reaction (DAB) (B1-2) OC125 antibody and (C1-2) OVTL3 anti-ovarian carcinoma antibody.
(A1): Cytospin preparation of malignant ascites with islets of reddish-stained tumor cells (1), bluish-stained larger mesothelial cells (2) and some of them contain vacuoles.
(A2): Ascites (Pap) with clustered small dense-stained tumor cells (1) and lighter-stained mesothelial cells with vacuoles ( ).
(B1): Ascitic tumor cell with large nucleus (3) and nucleolus with swollen mitochondria (4). Cell membrane and microvilli of tumor cell are coated with a thin electron-dense layer of DAB reaction product of OC125 antibody (→).
(B2): Higher magnification reveals numerous microvilli stained with OC125 antibody (→).
(C1): A cluster of three ascitic tumor cells shows membrane-bound OVTL3 reactivity (→) between the cells as well as at the free surface of top cell. Nuclei (3) and swollen mitochondria.
(C2): Higher magnification of electron-dense reaction product on epitopes (→) in membrane and microvilli. Cytoplasm with rough endoplasmic reticulum, mitochondria (4) and part of nucleus (3) remains unstained.
Background: See also Background POJA-L1476+L1760+L1770+L1750. The OC125 (MUC 16) antibody recognizes an epitope on a molecule called Cancer Antigen 125 (CA125) a.o. in tumor cell membranes. It is directed against a glycoprotein produced by human epithelial ovarian cancer cells. The antibody OVTL3 was generated against ovarian carcinoma cells and detect for the greater part cell membrane-bound epitopes of the carcinoma cells in the primary tumor as well as in distant metastases. The antibody was also applied for immunoscintigraphy. However cytoplasmic epitopes might be lost in suspended tumor cells (e.g. ascites).
Keywords/Mesh: female reproductive organs, ovary, OC125 antibody, OVTL3 antibody, ascites, histology, pathology, electron microscopy, female genitalia, ovarian neoplasms, papillary serous cystadenocarcinoma, ovarian tumor antigens, histology, POJA collection