12.2.4.1 POJA-L4337+2615+La0111+La0127+3451
Title: Reissner membrane in the cochlea of the inner ear
Description:
(A): Cochlea, stain toluidine blue, 4 days postnatal, rat. Survey of the cochlea. (1) Scala vestibuli (perilymph).
(2) Ductus cochlearis (endolymph). (3) Scala tympani (perilymph).
(B): Ductus cochlearis, immunoperoxidase staining with AEC and antibodies against collagen IV, 4 days postnatal rat.
The collagen IV is found in the lamina basilaris of the organ of Corti, in the area of the cochlear nerve (5), adjacent to the stria
vascularis (6), but also in the sandwich of the Reissner’s membrane (4) and the spiral limbus (7).
(C): Ductus cochlearis, stain Azan, guinea pig. Reissner’s membrane, which is composed of two layers of flattened epithelium, separated by a basement membrane. Its structure suggests that its function is transport of fluid and electrolytes to regulate Na+ homeostasis.
(D): Scheme electron microscopy Reissner's membrane, human.
(E): Reissner's membrane, electron micrograph, rat (By courtesy of E. Tonnaer PhD, Department of Otorhinolaryngology, Radboud university medical center, Nijmegen, The Netherlands)
(D, E) The internal epithelial cell layer of the Reissner’s membrane facing the endolymphatic space is continuous with that of the interdental cells. The external side is covered with a discontinuous layer of flattened mesothelial cells (D, 8) facing the perilymphatic space of the scala vestibuli.
The cochlear endolymph has a high-K+ and low-Na+ composition. Epithelial cells of the Reissner’s membrane and outer sulcus cells are reported to contribute to endolymphatic homeostasis by absorbing Na+ involving the expressed P2Y receptor (subtype 4).
The latter is a metabotropic receptor which regulates the activity of amioride-sensitive Na+ channels in the aforementioned
epithelial cells. It is reported that transporting Na+ ions out of the endolymph via apical Na+ channels to the perilymph occurs in response to noise exposure.
Keywords/Mesh: inner ear, cochlea, perilymph, Reissner’s membrane, endolymph, Na+ homeostasis, histology, electron microscopy,
POJA collection
Title: Reissner membrane in the cochlea of the inner ear
Description:
(A): Cochlea, stain toluidine blue, 4 days postnatal, rat. Survey of the cochlea. (1) Scala vestibuli (perilymph).
(2) Ductus cochlearis (endolymph). (3) Scala tympani (perilymph).
(B): Ductus cochlearis, immunoperoxidase staining with AEC and antibodies against collagen IV, 4 days postnatal rat.
The collagen IV is found in the lamina basilaris of the organ of Corti, in the area of the cochlear nerve (5), adjacent to the stria
vascularis (6), but also in the sandwich of the Reissner’s membrane (4) and the spiral limbus (7).
(C): Ductus cochlearis, stain Azan, guinea pig. Reissner’s membrane, which is composed of two layers of flattened epithelium, separated by a basement membrane. Its structure suggests that its function is transport of fluid and electrolytes to regulate Na+ homeostasis.
(D): Scheme electron microscopy Reissner's membrane, human.
(E): Reissner's membrane, electron micrograph, rat (By courtesy of E. Tonnaer PhD, Department of Otorhinolaryngology, Radboud university medical center, Nijmegen, The Netherlands)
(D, E) The internal epithelial cell layer of the Reissner’s membrane facing the endolymphatic space is continuous with that of the interdental cells. The external side is covered with a discontinuous layer of flattened mesothelial cells (D, 8) facing the perilymphatic space of the scala vestibuli.
The cochlear endolymph has a high-K+ and low-Na+ composition. Epithelial cells of the Reissner’s membrane and outer sulcus cells are reported to contribute to endolymphatic homeostasis by absorbing Na+ involving the expressed P2Y receptor (subtype 4).
The latter is a metabotropic receptor which regulates the activity of amioride-sensitive Na+ channels in the aforementioned
epithelial cells. It is reported that transporting Na+ ions out of the endolymph via apical Na+ channels to the perilymph occurs in response to noise exposure.
Keywords/Mesh: inner ear, cochlea, perilymph, Reissner’s membrane, endolymph, Na+ homeostasis, histology, electron microscopy,
POJA collection