7.2 ​POJA-L1650+1648+1646. Cervix and HPV insitu hybridization (ectocervix, human, adult)
7.2 POJA-L1650+1648+1646
Title: Cervix and HPV insitu hybridization (ectocervix, human, adult)
Description: Stain: (A, B) antikeratin antibody immunoperoxidase staining with diaminobenzidin reaction (DAB)and hematoxylin counterstaining; (C) in situ hybridization of HPV. (C): ISH HPV (human papilloma virus).
(A): Polykeratin expression in (NKSSE) non-keratinised stratified squamous epithelium of the ectocervix. All cervical cells (1) express keratin while the stroma (2) of the proper lamina is negative. Note that intermediate and superficial cells appear swollen and transparent due to the unstained content of glycogen.
(B): There is no cytokeratin 7 (antibody OVTL 12-30) expression in the NKSSE (1) and proper lamina (2). There is some unspecific background staining due to fixation procedures. Generally keratin 7 is not expressed in complex epithelium but is variably present in simple epithelium. The epithelial cells remain negative for keratin-7 (OVTL12/30).
(C): Nucleic acid in situ hybridization for HPV nucleic acids of NKSSE of another ectocervix specimen. Brown-black stain indicates HPV DNA in superficial cells (**). (1) intermediate, parabasal and basal cells show no incorporation of HPV. (2) proper lamina. (By courtesy of F. Smedts MD PhD, former pathologist in the Department of Pathology, Radboud university medical center, Nijmegen, The Netherlands).
Background: Human Papilloma Virus (HPV) plays an important role in cervical oncogenesis. Among others HPV DNA is detected by hybridization techniques in up to 85% of cervical cancers. But there is also evidence that HPV is not the only factor although in the reproductive years a high percentage of youngsters is infected with one or more HPV types , but only a few develop cervical cancer. Immune status, nutrition, contraceptives, smoking, p53 mutations are also cofactors that codetermine whether a HPV infection remains latent or results in a CIN (Cervical Intraepithelial Neoplasia) or even progresses to cervical cancer.
Keywords/Mesh: female reproductive organs, ectocervix, HPV papilloma virus, ISH, in situ hybridization, keratin, cervix, histology, POJA collection.
Title: Cervix and HPV insitu hybridization (ectocervix, human, adult)
Description: Stain: (A, B) antikeratin antibody immunoperoxidase staining with diaminobenzidin reaction (DAB)and hematoxylin counterstaining; (C) in situ hybridization of HPV. (C): ISH HPV (human papilloma virus).
(A): Polykeratin expression in (NKSSE) non-keratinised stratified squamous epithelium of the ectocervix. All cervical cells (1) express keratin while the stroma (2) of the proper lamina is negative. Note that intermediate and superficial cells appear swollen and transparent due to the unstained content of glycogen.
(B): There is no cytokeratin 7 (antibody OVTL 12-30) expression in the NKSSE (1) and proper lamina (2). There is some unspecific background staining due to fixation procedures. Generally keratin 7 is not expressed in complex epithelium but is variably present in simple epithelium. The epithelial cells remain negative for keratin-7 (OVTL12/30).
(C): Nucleic acid in situ hybridization for HPV nucleic acids of NKSSE of another ectocervix specimen. Brown-black stain indicates HPV DNA in superficial cells (**). (1) intermediate, parabasal and basal cells show no incorporation of HPV. (2) proper lamina. (By courtesy of F. Smedts MD PhD, former pathologist in the Department of Pathology, Radboud university medical center, Nijmegen, The Netherlands).
Background: Human Papilloma Virus (HPV) plays an important role in cervical oncogenesis. Among others HPV DNA is detected by hybridization techniques in up to 85% of cervical cancers. But there is also evidence that HPV is not the only factor although in the reproductive years a high percentage of youngsters is infected with one or more HPV types , but only a few develop cervical cancer. Immune status, nutrition, contraceptives, smoking, p53 mutations are also cofactors that codetermine whether a HPV infection remains latent or results in a CIN (Cervical Intraepithelial Neoplasia) or even progresses to cervical cancer.
Keywords/Mesh: female reproductive organs, ectocervix, HPV papilloma virus, ISH, in situ hybridization, keratin, cervix, histology, POJA collection.