14.1 POJA-L6212-BB Typing of muscle fibres with ATPase histochemistry (human)
14.1 POJA-L6212-BB Typing of muscle fibres with ATPase histochemistry (human)
(The image in 14.1 POJA-L6212-BB ‘Typing of muscle fibres with ATPase histochemistry’ is the original composition of H. ter Laak PhD that could be reproduced in full with his permission, including his description. We are grateful to him for this gesture. Dr. H. ter Laak is a retired staff member of the Section Neuropathology, Department of Pathology, Radboud university medical center, Nijmegen, the Netherlands)
Title: Typing of muscle fibres with ATPase histochemistry (human)
Description:
ATPase was assayed at different pH’s on serial sections of skeletal muscles.
(A): At pH 10.3 showing type I and type II fibres at slightly different staining level.
(B): At pH 4.2 showing a strong difference in staining level between type I and type II.
(C): At pH 4.6 shows additional staining difference between type IIA and IIB.
See also:
Background:
The use of the method of ATPase enzyme histochemistry is a standard technique for classifying fibre types. Most skeletal muscles reveal a checkerboard pattern of light- and dark-stained ATPase activities. Type I fibres have a high oxidative/low glycolytic activity. Type II fibres have a low oxidative/high glycolytic activity. However, a subtype of type II fibres (IIB) is moderate oxidative/high glycolytic.
Physiologically there are 3 main types of fibres: fast twitch, fatigue sensitive (FF); fast twitch, fatigue resistant (FR); slow twitch, fatigue resistant (S). Fatigue resistance is correlated to oxidative capacity and the number of mitochondria. Hence type I fibres are slow twitch/oxidative (SO); type IIB fibres are fast twitch/glycolytic (FG); type IIA fibres are fast twitch/oxidative glycolytic (FOG).
At present, antibodies against myosin heavy chain isoforms are also used to classify fibre types into fast myosin and slow myosin fibres. Normal mature muscle cells express only one heavy chain myosin isoform.
Keywords/Mesh: locomotor system, skeletal muscle, striated muscle, oxidative activity, glycolytic activity, slow twitch, fast twitch, myosin, ATPase, type I fibre, type II fibre, histology, POJA collection
(The image in 14.1 POJA-L6212-BB ‘Typing of muscle fibres with ATPase histochemistry’ is the original composition of H. ter Laak PhD that could be reproduced in full with his permission, including his description. We are grateful to him for this gesture. Dr. H. ter Laak is a retired staff member of the Section Neuropathology, Department of Pathology, Radboud university medical center, Nijmegen, the Netherlands)
Title: Typing of muscle fibres with ATPase histochemistry (human)
Description:
ATPase was assayed at different pH’s on serial sections of skeletal muscles.
(A): At pH 10.3 showing type I and type II fibres at slightly different staining level.
(B): At pH 4.2 showing a strong difference in staining level between type I and type II.
(C): At pH 4.6 shows additional staining difference between type IIA and IIB.
See also:
- 14.1 POJA-L6212-CC Table muscle fibre typing
Background:
The use of the method of ATPase enzyme histochemistry is a standard technique for classifying fibre types. Most skeletal muscles reveal a checkerboard pattern of light- and dark-stained ATPase activities. Type I fibres have a high oxidative/low glycolytic activity. Type II fibres have a low oxidative/high glycolytic activity. However, a subtype of type II fibres (IIB) is moderate oxidative/high glycolytic.
Physiologically there are 3 main types of fibres: fast twitch, fatigue sensitive (FF); fast twitch, fatigue resistant (FR); slow twitch, fatigue resistant (S). Fatigue resistance is correlated to oxidative capacity and the number of mitochondria. Hence type I fibres are slow twitch/oxidative (SO); type IIB fibres are fast twitch/glycolytic (FG); type IIA fibres are fast twitch/oxidative glycolytic (FOG).
At present, antibodies against myosin heavy chain isoforms are also used to classify fibre types into fast myosin and slow myosin fibres. Normal mature muscle cells express only one heavy chain myosin isoform.
Keywords/Mesh: locomotor system, skeletal muscle, striated muscle, oxidative activity, glycolytic activity, slow twitch, fast twitch, myosin, ATPase, type I fibre, type II fibre, histology, POJA collection