9.5 POJA-L2892+La0238+2893+3535
Title: Adrenalin and noradrenalin cells (I) in the medulla of the rat adrenal gland
Description:
(A, C): Immunohistochemical staining with immunoperoxidase and DAB (diaminobenzidin) using anti-epinephrine (adrenalin) antibodies (A) and anti-norepinephrine (noradrenalin) antibodies (C) on frozen sections of the rat adrenal gland.
(B, D): Electron micrographs of adrenalin cells, with large dense-core granules in (B) and of noradrenalin cells in (D).
Adrenalin and noradrenalin are produced and secreted by two different cell types. The adrenalin cells (B) possess uniform grey granules which are moderate electron-dense, while noradrenalin cells (D) have granules with an irregular electron-dense center and
a lucent outer rim.
Background: The amino acid tyrosine is taken up in the cell and processed into dopamine in the cytoplasm of the cell.
The content of the granules largely comprise a matrix including the proteins chromogranin and encephalin, plus ATP and the enzyme dopamine β-hydroxylase for the conversion of dopamine into noradrenalin.
The conversion of noradrenalin into adrenalin can occur upon leaving the granule for being methylated in the cytoplasm by the enzyme phenylethanolamin-N-methyltransferase. I
n humans 80% of these cells are involved in adrenalin production.
Keywords/Mesh: adrenal gland,, medulla, adrenalin, noradrenalin, histology, electron microscopy, POJA collection
Title: Adrenalin and noradrenalin cells (I) in the medulla of the rat adrenal gland
Description:
(A, C): Immunohistochemical staining with immunoperoxidase and DAB (diaminobenzidin) using anti-epinephrine (adrenalin) antibodies (A) and anti-norepinephrine (noradrenalin) antibodies (C) on frozen sections of the rat adrenal gland.
(B, D): Electron micrographs of adrenalin cells, with large dense-core granules in (B) and of noradrenalin cells in (D).
Adrenalin and noradrenalin are produced and secreted by two different cell types. The adrenalin cells (B) possess uniform grey granules which are moderate electron-dense, while noradrenalin cells (D) have granules with an irregular electron-dense center and
a lucent outer rim.
Background: The amino acid tyrosine is taken up in the cell and processed into dopamine in the cytoplasm of the cell.
The content of the granules largely comprise a matrix including the proteins chromogranin and encephalin, plus ATP and the enzyme dopamine β-hydroxylase for the conversion of dopamine into noradrenalin.
The conversion of noradrenalin into adrenalin can occur upon leaving the granule for being methylated in the cytoplasm by the enzyme phenylethanolamin-N-methyltransferase. I
n humans 80% of these cells are involved in adrenalin production.
Keywords/Mesh: adrenal gland,, medulla, adrenalin, noradrenalin, histology, electron microscopy, POJA collection