13.1 POJA-L4719+4594+4561+4564+4596+4563+4565+4572 Elastic artery: light and electron microscopy
mooth13.1 POJA-L4719+4594+4561+4564+4596+4563+4565+4572.
Title: Elastic artery: light and electron microscopy
Description:
(A): Scheme elastic artery. (2) Intima. (3) Media. (4) Adventitia with vasa vasorum.
(B): Common carotid artery, haematoxylin-orcein, human. (1) Lumen. (2) Intima. (3) Media. (4) Adventitia with vasa vasorum.
(C1-2): Aorta/radial artery, orcein, human. Compare to (B): lumen of vessel (1), blood vessel with intima (2), media (3), adventitia (4). The tunica intima is the innermost tunic (=layer) and consist of a thin endothelium and a thin subendothelial connective tissue layer followed by a thin fenestrated layer of elastic material (internal elastic lamina or IEL) at the border of the next layer. This middle tunic (=tunica media) contains smooth muscle cells, extracellular matrix (ECM), collagen fibres and elastic material deposited in fenestrated membranes. The outer layer (=tunica externa or adventitia) is composed of connective tissue (collagen fibres and considerable amount of elastic material). Between the medial and outer layer, a thin external elastic lamina (EEL) is mostly present.
The differences between an elastic artery (aorta) and a muscular artery (radial artery) is demonstrated by staining elastic fibers with orcein.
(C1) A conducting artery (or elastic artery) e.g. aorta, common carotid artery contains numerous interwoven elastic fibres within concentric elastic membranes with perforations (fenestrations) in the media, and scattered elastic fibres (C1, 4) in the adventitia. The elastic membranes are interconnected by smooth muscle cells in an extracellular matrix (ECM) with elastic fibres and collagen.
(C2) A distributing artery (or muscular artery) e.g. radial artery, femoral artery possesses a distinct internal (arrow) elastic lamina (IEL) between the intima and media, a muscular media with finely distributed elastic fibres between the smooth muscle cells, subsequently an external (arrowhead) elastic lamina (EEL) followed by numerous coarse elastic fibres in the adventitia (C2, 4).
(D1-2): D1: Carotid artery, Azan, human. (D2): Aorta, orcein without counterstain, human. Just as the aorta its branch is the carotid artery a conducting artery with numerous elastic laminae. In both their middle tunics (media) branched smooth muscle cells (arrows point to nuclei) are present and interlink between elastic membranes which are visualised specifically in D2 by orcein staining. The muscle cells are found slightly orientated in a herringbone pattern.
(E): Elastic artery (carotid artery), EM, rat. (2) Endothelium with basal lamina. (3) Subendothelial space with collagen fibrils. (4) Internal elastic membrane (IEL). (5) Branched myocytes with nuclei (smooth muscle cells or SMC) embedded between collagen fibrils. (6) Elastic lamina and close association with branched myocytes.
(F1-2): Aorta, orcein without counterstain, human. All the elastic material of the media is mainly represented in ca. 50-75 concentric fenestrated membranes (laminae) or sheaths. The fact that we are dealing with true membranes and not with fibres is demonstrated by their identical appearance in longitudinal section (F1) as well as in cross-section (F2).
(G): Media of aorta, resorcin-fuchsin and modified light green, human. A part of the aortic media shows many distinct darker red-stained elastic membranes (large arrow) while branched smooth muscle cells (1) appear embedded in light-stained ECM (matrix, 2) with collagen interlinked between elastic membranes. Note herringbone pattern (double-headed arrows) of SMC orientation.
(H): Adventitia aorta, resorcin-fuchsin and modified light green, human. Less brown-stained elastic material in adventitia with small vessels (arteries and vein) for oxygen supply.
Background: An elastic (or conducting) artery contains apart from the intima a media with numerous interwoven elastic fibres within concentric elastic fenestrated membranes, and scattered elastic fibres in the adventitia. The elastic membranes are interconnected by smooth muscle cells (SMC) in an extracellular matrix (ECM) with elastic fibres and collagen.
Keywords/Mesh: cardiovascular system, vascularisation, blood vessel, conducting artery, elastic artery , elastic lamella, vasa vasorum, aorta, carotid artery, histology, POJA collection
Title: Elastic artery: light and electron microscopy
Description:
(A): Scheme elastic artery. (2) Intima. (3) Media. (4) Adventitia with vasa vasorum.
(B): Common carotid artery, haematoxylin-orcein, human. (1) Lumen. (2) Intima. (3) Media. (4) Adventitia with vasa vasorum.
(C1-2): Aorta/radial artery, orcein, human. Compare to (B): lumen of vessel (1), blood vessel with intima (2), media (3), adventitia (4). The tunica intima is the innermost tunic (=layer) and consist of a thin endothelium and a thin subendothelial connective tissue layer followed by a thin fenestrated layer of elastic material (internal elastic lamina or IEL) at the border of the next layer. This middle tunic (=tunica media) contains smooth muscle cells, extracellular matrix (ECM), collagen fibres and elastic material deposited in fenestrated membranes. The outer layer (=tunica externa or adventitia) is composed of connective tissue (collagen fibres and considerable amount of elastic material). Between the medial and outer layer, a thin external elastic lamina (EEL) is mostly present.
The differences between an elastic artery (aorta) and a muscular artery (radial artery) is demonstrated by staining elastic fibers with orcein.
(C1) A conducting artery (or elastic artery) e.g. aorta, common carotid artery contains numerous interwoven elastic fibres within concentric elastic membranes with perforations (fenestrations) in the media, and scattered elastic fibres (C1, 4) in the adventitia. The elastic membranes are interconnected by smooth muscle cells in an extracellular matrix (ECM) with elastic fibres and collagen.
(C2) A distributing artery (or muscular artery) e.g. radial artery, femoral artery possesses a distinct internal (arrow) elastic lamina (IEL) between the intima and media, a muscular media with finely distributed elastic fibres between the smooth muscle cells, subsequently an external (arrowhead) elastic lamina (EEL) followed by numerous coarse elastic fibres in the adventitia (C2, 4).
(D1-2): D1: Carotid artery, Azan, human. (D2): Aorta, orcein without counterstain, human. Just as the aorta its branch is the carotid artery a conducting artery with numerous elastic laminae. In both their middle tunics (media) branched smooth muscle cells (arrows point to nuclei) are present and interlink between elastic membranes which are visualised specifically in D2 by orcein staining. The muscle cells are found slightly orientated in a herringbone pattern.
(E): Elastic artery (carotid artery), EM, rat. (2) Endothelium with basal lamina. (3) Subendothelial space with collagen fibrils. (4) Internal elastic membrane (IEL). (5) Branched myocytes with nuclei (smooth muscle cells or SMC) embedded between collagen fibrils. (6) Elastic lamina and close association with branched myocytes.
(F1-2): Aorta, orcein without counterstain, human. All the elastic material of the media is mainly represented in ca. 50-75 concentric fenestrated membranes (laminae) or sheaths. The fact that we are dealing with true membranes and not with fibres is demonstrated by their identical appearance in longitudinal section (F1) as well as in cross-section (F2).
(G): Media of aorta, resorcin-fuchsin and modified light green, human. A part of the aortic media shows many distinct darker red-stained elastic membranes (large arrow) while branched smooth muscle cells (1) appear embedded in light-stained ECM (matrix, 2) with collagen interlinked between elastic membranes. Note herringbone pattern (double-headed arrows) of SMC orientation.
(H): Adventitia aorta, resorcin-fuchsin and modified light green, human. Less brown-stained elastic material in adventitia with small vessels (arteries and vein) for oxygen supply.
Background: An elastic (or conducting) artery contains apart from the intima a media with numerous interwoven elastic fibres within concentric elastic fenestrated membranes, and scattered elastic fibres in the adventitia. The elastic membranes are interconnected by smooth muscle cells (SMC) in an extracellular matrix (ECM) with elastic fibres and collagen.
Keywords/Mesh: cardiovascular system, vascularisation, blood vessel, conducting artery, elastic artery , elastic lamella, vasa vasorum, aorta, carotid artery, histology, POJA collection