7.1 POJA-L1912+1913+1914. Papillary serous cystadenocarcinoma of ovary (human, adult)
7.1 POJA-L1912+1913+1914
Title: Papillary serous cystadenocarcinoma of ovary (human, adult)
Description: Case nr. p577. Stain: (A) Hematoxylin-eosin (Weigert); (B) Fluorescent isothiocyanate (FITC)-labeled OVTL12/5 (CK 5, 7, 14, 19) antikeratin antibody; (C) FITC-labeled OC125 antibody (immunofluorescence, frozen section).
(A): Invaded tubuli of tumor cells with formation of acini (1). Variation in nuclear hyperchromatism, mitoses and nuclear atypia. Fibrocellular stroma (2).
(B): Tumor cells (1) only display specific reactivity for broad spectrum keratin. There is abundant expression of keratin. Stroma negative (2).
(C): In contrast to keratin expression not all tumor cells (1) react heterogeneously strong. Intensity of linear fluorescence along tumor cell membranes varies between individual cells. However stroma remains negative (2).
Background: See also Background POJA-L1476+L1760+L1770+L1750. Solid areas of the tumor are composed of closely packed papillae. Destructive infiltrative stromal invasion is evident as the tumor ingrowth is diffuse or as solid cell sheets. Extreme degrees of nuclear atypia indicate progress toward undifferentiated tumor cells. Normal simple lining epithelia as ovarian surface epithelial cells and epithelium of most mucous or serous glands generally express broad spectrum keratin OVTL12/5. During abnormal differentiation of these epithelial cells OVTL12/5 expression remains preserved. Individual tumor cells of well-differentiated serous adenocarcinomas metastasize by invasion or via blood vessels. Application of this broad spectrum keratin allows histological detection of solitary tumor cells more easily and convincingly. The OC125 (MUC 16) antibody recognizes an epitope on a molecule called Cancer Antigen 125 (CA125) a.o. in tumor cell membranes. It is directed against a glycoprotein produced by human epithelial ovarian cancer cells. The antigenic determinant CA125 is expressed on more than 80% of all non-mucinous epithelial ovarian tumors. It does not react with mucinous ovarian tumors. It is not a tumor-specific antigen, it presence was also demonstrated in fetal as well as in adult normal tissues derived from coelomic epithelium.
Keywords/Mesh: female reproductive organs, ovary, OC125 antibody, OVTL12/5 anti-keratin 5/7 /14/19 antibody, keratin, ovarian neoplasms, papillary serous cystadenocarcinoma, immunofluorescence, histology, POJA collection
Title: Papillary serous cystadenocarcinoma of ovary (human, adult)
Description: Case nr. p577. Stain: (A) Hematoxylin-eosin (Weigert); (B) Fluorescent isothiocyanate (FITC)-labeled OVTL12/5 (CK 5, 7, 14, 19) antikeratin antibody; (C) FITC-labeled OC125 antibody (immunofluorescence, frozen section).
(A): Invaded tubuli of tumor cells with formation of acini (1). Variation in nuclear hyperchromatism, mitoses and nuclear atypia. Fibrocellular stroma (2).
(B): Tumor cells (1) only display specific reactivity for broad spectrum keratin. There is abundant expression of keratin. Stroma negative (2).
(C): In contrast to keratin expression not all tumor cells (1) react heterogeneously strong. Intensity of linear fluorescence along tumor cell membranes varies between individual cells. However stroma remains negative (2).
Background: See also Background POJA-L1476+L1760+L1770+L1750. Solid areas of the tumor are composed of closely packed papillae. Destructive infiltrative stromal invasion is evident as the tumor ingrowth is diffuse or as solid cell sheets. Extreme degrees of nuclear atypia indicate progress toward undifferentiated tumor cells. Normal simple lining epithelia as ovarian surface epithelial cells and epithelium of most mucous or serous glands generally express broad spectrum keratin OVTL12/5. During abnormal differentiation of these epithelial cells OVTL12/5 expression remains preserved. Individual tumor cells of well-differentiated serous adenocarcinomas metastasize by invasion or via blood vessels. Application of this broad spectrum keratin allows histological detection of solitary tumor cells more easily and convincingly. The OC125 (MUC 16) antibody recognizes an epitope on a molecule called Cancer Antigen 125 (CA125) a.o. in tumor cell membranes. It is directed against a glycoprotein produced by human epithelial ovarian cancer cells. The antigenic determinant CA125 is expressed on more than 80% of all non-mucinous epithelial ovarian tumors. It does not react with mucinous ovarian tumors. It is not a tumor-specific antigen, it presence was also demonstrated in fetal as well as in adult normal tissues derived from coelomic epithelium.
Keywords/Mesh: female reproductive organs, ovary, OC125 antibody, OVTL12/5 anti-keratin 5/7 /14/19 antibody, keratin, ovarian neoplasms, papillary serous cystadenocarcinoma, immunofluorescence, histology, POJA collection