Title : Heparan sulfates in the terminal sac-period of the fetal mouse lung (1)
Fluorescence microscopy with anti-heparan sulphate antibody (a phage-display antibody, LKiv69). Heparan sulfates belong to the group of the glycosaminoglycans (GAGs). Strong fluorescence visualizes the course of all basement membranes, especially around the bronchioles (B) and around the bundles of smooth muscles (↓). Both subendothelially within the muscular pulmonary artery (PA) and in the adventia fluorescence shows the characteristic double elastic layer of a larger pulmonary artery. In between the positive structures unstained lymph vessels (Lv) without basement membranes are visible. Note at (A) the reactive lining of the air spaces (future alveoli).
Title : Heparan sulfates in the terminal sac-period of the fetal mouse lung (2)
Ditto fluorescence microscopy. Strong fluorescence visualizes the course of all basement membranes, especially around the bronchioles (B), subendothelially within the pulmonary artery (PA) and small branches. The lining of the air spaces (A) and capillaries within the thick septa (with non-inflatable alveoli) are also well-stained. Note negative reacting lymph vessels (Lv) (no basement membrane).
Title: Laminin in the canalicular period of the embryonal mouse lung
Fluorescence microscopy with anti-laminin antibody. Laminin is a component of the basement membrane. Strong fluorescence visualizes the course of all basement membranes, especially around the bronchioles (B) and their branches in the developing lung tissue. In between the distal air spaces (tubes, T) lined by cuboidal epithelium and capillary basement membranes are also stained. At (↓) positive blood vessel within the pleura (PL↔).
Background: Heparan sulfates are linear polysaccharides that belongs to the group of the glycosaminoglycans (GAGs).
The sulphated saccharide domains provide numerous docking sites for protein ligands. These GAGs are found associated with basement membranes as shown here. GAG-expression pattern changes during tumour development.
The anti-laminin antibody is used as a marker for intact or disrupted basement membranes of epithelial structures and tumour metastasis.
Keywords/Mesh: respiratory system, lung, embryo, fetus, canalicular period, terminal sac period, pulmonal artery, lymph vessel, bronchiole, smooth muscle, air space, alveolus, capillary, basement membrane, heparan sulfate, laminin, histology, embryology, POJA collection