POJA-L234+195+1856
Title: Normal breast in pregnancy and after cessation of lactation (human)
Description: Stain: (A, B) Azan; (C) CK 7 (OVTL12-30) antikeratin antibody immunoperoxidase staining with diaminobenzidin reaction (DAB) and hematoxylin counterstaining.
(A): Early pregnancy. Survey of enlarged lobules (1) and extralobular terminal ducts (2). Few lobules contain groups of dilated alveoli (or acini) (**). Blue-stained extralobular fibrocollagenous tissue (3).
(B): Cessation of lactation. Survey shows regression of atrophying lobules (1) that become infiltrated by adipocytes (4). The tubuloalveoli become narrower and their number decreases. Most intra- and interlobular ducts (3) are dilated and contain remaining milk particles and cell debris that will be phagocytized and discharged.
(C): Cessation of lactation. Monoclonal antibody to keratin 7 stains all tubuloalveoli (1), intralobular (1a) and interlobular ducts with remnants of secretion (3). A mixture of still enlarged alveoli (or acini) and small terminal duct lobular units are present. Replacement by adipocytes at (4) and thickened fibrocollagenous stroma (5) that remains keratin 7- negative.
Background: Following the cessation of lactation the mammary gland goes through a tissue remodeling process the so-called involution. The amount of glandular structures decreases and the adipose tissue compartment takes up a much larger proportion of the tissue. Regressive changes in mammary glands are induced by increasing levels of estrogens and reestablishment of the menstrual cycle. The total breast size is diminished remarkably.
Keywords/Mesh: breast, mammary glands, histology, POJA collection, cytokeratin 7, lactation, regression, pregnancy
Title: Normal breast in pregnancy and after cessation of lactation (human)
Description: Stain: (A, B) Azan; (C) CK 7 (OVTL12-30) antikeratin antibody immunoperoxidase staining with diaminobenzidin reaction (DAB) and hematoxylin counterstaining.
(A): Early pregnancy. Survey of enlarged lobules (1) and extralobular terminal ducts (2). Few lobules contain groups of dilated alveoli (or acini) (**). Blue-stained extralobular fibrocollagenous tissue (3).
(B): Cessation of lactation. Survey shows regression of atrophying lobules (1) that become infiltrated by adipocytes (4). The tubuloalveoli become narrower and their number decreases. Most intra- and interlobular ducts (3) are dilated and contain remaining milk particles and cell debris that will be phagocytized and discharged.
(C): Cessation of lactation. Monoclonal antibody to keratin 7 stains all tubuloalveoli (1), intralobular (1a) and interlobular ducts with remnants of secretion (3). A mixture of still enlarged alveoli (or acini) and small terminal duct lobular units are present. Replacement by adipocytes at (4) and thickened fibrocollagenous stroma (5) that remains keratin 7- negative.
Background: Following the cessation of lactation the mammary gland goes through a tissue remodeling process the so-called involution. The amount of glandular structures decreases and the adipose tissue compartment takes up a much larger proportion of the tissue. Regressive changes in mammary glands are induced by increasing levels of estrogens and reestablishment of the menstrual cycle. The total breast size is diminished remarkably.
Keywords/Mesh: breast, mammary glands, histology, POJA collection, cytokeratin 7, lactation, regression, pregnancy