7.2 POJA-L1641+1726+1727
Title: Cervix (endocervix, human, adult)
Description: Stain: (A) Azan; (B, C) antikeratin 7 antibody (OVTL 12-30) immunoperoxidase staining with diaminobenzidin reaction (DAB) and hematoxylin counterstaining (middle/right).
(A): Survey of endocervix (1) and ectocervix or portio vaginalis (2) with non-keratinised stratified squamous epithelium and proper lamina (3); (4) cervical glands and crypts in proper lamina (3); (5) myometrium with a considerable amount of connective tissue (blue) and blood vessels; (6) denoted a small nabothian cyst and several cervical glands in an area of squamous metaplasia; (*---*) is transformation zone.
(B): Endocervix with branched tubular cervical glands. The secretory epithelium is continuous with the surface-lining of the endocervical canal and both express keratin 7 (dark brown).
(C): The (NKSSE) non-keratinised stratified squamous epithelium (7) in squamous metaplasia of the cervix is negative for keratin 7 staining. This means that complete maturation results in loss of keratin 7 expression. The endocervical glands (8), however, show strong cytokeratin 7 expression.
Background: Cytokeratin 7 is generally expressed by simple epithelia c.q. the epithelium of most mucous or serous glands. Stratified squamous epithelia mostly are negative. After puberty the former alkaline pH of vagina and cervix becomes acidic due to glycogen breakdown (bacteria) in the NKSSE. The size and position of squamocolumnar junction is variable and that junction normally is found in the area of the external os. Upon exposure to the acidic environment squamous metaplasia is induced and new squamous epithelium is found in the transformation zone previously occupied by columnar endocervical epithelium. The consequence of squamous metaplasia is that the apertures of some deep crypts of the endocervical glands become obliterated. Consequently, the mucus accumulates resulting in the formation of spherical cysts (Nabothnii cysts) up to 5 mm or more in diameter.The cysts are lined by flattened glandular epithelium.
Keywords/Mesh: female reproductive organs, uterus, ectocervix, portio vaginalis, cervix uteri, cytokeratin 7, cervical glands, non-keratinised stratified squamous epithelium, histology, POJA collection.
Title: Cervix (endocervix, human, adult)
Description: Stain: (A) Azan; (B, C) antikeratin 7 antibody (OVTL 12-30) immunoperoxidase staining with diaminobenzidin reaction (DAB) and hematoxylin counterstaining (middle/right).
(A): Survey of endocervix (1) and ectocervix or portio vaginalis (2) with non-keratinised stratified squamous epithelium and proper lamina (3); (4) cervical glands and crypts in proper lamina (3); (5) myometrium with a considerable amount of connective tissue (blue) and blood vessels; (6) denoted a small nabothian cyst and several cervical glands in an area of squamous metaplasia; (*---*) is transformation zone.
(B): Endocervix with branched tubular cervical glands. The secretory epithelium is continuous with the surface-lining of the endocervical canal and both express keratin 7 (dark brown).
(C): The (NKSSE) non-keratinised stratified squamous epithelium (7) in squamous metaplasia of the cervix is negative for keratin 7 staining. This means that complete maturation results in loss of keratin 7 expression. The endocervical glands (8), however, show strong cytokeratin 7 expression.
Background: Cytokeratin 7 is generally expressed by simple epithelia c.q. the epithelium of most mucous or serous glands. Stratified squamous epithelia mostly are negative. After puberty the former alkaline pH of vagina and cervix becomes acidic due to glycogen breakdown (bacteria) in the NKSSE. The size and position of squamocolumnar junction is variable and that junction normally is found in the area of the external os. Upon exposure to the acidic environment squamous metaplasia is induced and new squamous epithelium is found in the transformation zone previously occupied by columnar endocervical epithelium. The consequence of squamous metaplasia is that the apertures of some deep crypts of the endocervical glands become obliterated. Consequently, the mucus accumulates resulting in the formation of spherical cysts (Nabothnii cysts) up to 5 mm or more in diameter.The cysts are lined by flattened glandular epithelium.
Keywords/Mesh: female reproductive organs, uterus, ectocervix, portio vaginalis, cervix uteri, cytokeratin 7, cervical glands, non-keratinised stratified squamous epithelium, histology, POJA collection.