5.4.3 POJA-L5041+2432-II.
Localization of heparan sulfate and aquaporin (V) in kidney
5.4.3 POJA-L5041+2432-II
Title: Localization of heparan sulfate and aquaporin (V) in kidney
Description:
(A): Scheme nephron, human.
(B):Kidney, rat: Immunofluorescence staining with FITC-labeled antibodies NS4F5 against a specific domain of heparan sulfate (HSPG) showing labeling in descending thin limbs (DTL) and some small blood vessels.
Generally, however, HSPG is present in all basal laminas and the interstitial capillaries.
(C): KIdney, rat: Immunofluorescence staining with Texas Red and antibodies AQP-1 against aquaporin (integral membrane pore proteins) of the water channels for water reabsorption. AQP-1 is positive for proximal convoluted tubule (PCT), proximal straight tubule (PST)
and the intermediate tubule (IT) until the distal straight tubule (DST or mTAL).
(D): KIdney, rat: The merge of (B) and (C) showing the limited coincidence of both labels.
(B, C, D, by courtesy of N.C. Smits PhD former postdoc of Department of Biochemistry and Department of Pulmonary Diseases,
Radboud university medical center, Nijmegen, The Netherlands).
(E): Electron micrograph of medulla kidney with cross-sections through capillaries (3), a thin limb (2), and the mTAL (1).
Keywords/Mesh: urinary system, kidney, heparan sulfate, aquaporin, thin limb, histology, electron microscopy, POJA collection
Title: Localization of heparan sulfate and aquaporin (V) in kidney
Description:
(A): Scheme nephron, human.
(B):Kidney, rat: Immunofluorescence staining with FITC-labeled antibodies NS4F5 against a specific domain of heparan sulfate (HSPG) showing labeling in descending thin limbs (DTL) and some small blood vessels.
Generally, however, HSPG is present in all basal laminas and the interstitial capillaries.
(C): KIdney, rat: Immunofluorescence staining with Texas Red and antibodies AQP-1 against aquaporin (integral membrane pore proteins) of the water channels for water reabsorption. AQP-1 is positive for proximal convoluted tubule (PCT), proximal straight tubule (PST)
and the intermediate tubule (IT) until the distal straight tubule (DST or mTAL).
(D): KIdney, rat: The merge of (B) and (C) showing the limited coincidence of both labels.
(B, C, D, by courtesy of N.C. Smits PhD former postdoc of Department of Biochemistry and Department of Pulmonary Diseases,
Radboud university medical center, Nijmegen, The Netherlands).
(E): Electron micrograph of medulla kidney with cross-sections through capillaries (3), a thin limb (2), and the mTAL (1).
Keywords/Mesh: urinary system, kidney, heparan sulfate, aquaporin, thin limb, histology, electron microscopy, POJA collection