7.1 POJA-L1206+1232+1847. Primordial/primary follicles in ovary (human, adult)
7.1 POJA-L1206-1232+L1847
Title: Primordial/primary follicles in ovary (human, adult)
Description: Stain: (A) Azocarmine G; (B) Azan; (C) Antikeratin 8 antibody (CAM 5.2) immunoperoxidase staining with diaminobenzidin reaction (DAB) and hematoxylin counterstaining. See also scheme in POJA-L1201+L1231.
(A, B): Two primordial follicles (1) and one intermediary primordial follicle (A, 2) are embedded in a specialized type of loose connective tissue. This so-called cellular connective tissue has a high cellularity of spindle-like fibroblast or stromal cells (3) associated with collagen fibers (blue in B); it is characteristic for the ovary. The oocytes in the primordial follicles (1) type 1 are surrounded by a single thin layer of flattened follicular (or pregranulosa) cells. Although the unilaminar intermediary primordial follicle (2) shows few flattened follicular cells, the majority, however, is low cuboidal. Note the distinct nucleolus in the oocyte. Generally the nucleus/cytoplasm ratio is the highest in the primordial follicle and decreases during maturity as the cytoplasm increases in volume.
(C): Follicular cells of type 1 as well as type 2 follicles are distinct cytokeratin-positive, and thus of epithelial nature while oocytes and stroma react negatively.
Keyword/Mesh: female reproductive organs, ovary, primordial follicle, oocyte ,stroma, female genitalia, keratin 8, CAM 5.2 antibody, azocarmine, histology, POJA collection
Title: Primordial/primary follicles in ovary (human, adult)
Description: Stain: (A) Azocarmine G; (B) Azan; (C) Antikeratin 8 antibody (CAM 5.2) immunoperoxidase staining with diaminobenzidin reaction (DAB) and hematoxylin counterstaining. See also scheme in POJA-L1201+L1231.
(A, B): Two primordial follicles (1) and one intermediary primordial follicle (A, 2) are embedded in a specialized type of loose connective tissue. This so-called cellular connective tissue has a high cellularity of spindle-like fibroblast or stromal cells (3) associated with collagen fibers (blue in B); it is characteristic for the ovary. The oocytes in the primordial follicles (1) type 1 are surrounded by a single thin layer of flattened follicular (or pregranulosa) cells. Although the unilaminar intermediary primordial follicle (2) shows few flattened follicular cells, the majority, however, is low cuboidal. Note the distinct nucleolus in the oocyte. Generally the nucleus/cytoplasm ratio is the highest in the primordial follicle and decreases during maturity as the cytoplasm increases in volume.
(C): Follicular cells of type 1 as well as type 2 follicles are distinct cytokeratin-positive, and thus of epithelial nature while oocytes and stroma react negatively.
Keyword/Mesh: female reproductive organs, ovary, primordial follicle, oocyte ,stroma, female genitalia, keratin 8, CAM 5.2 antibody, azocarmine, histology, POJA collection