2.1 POJA-L935. The effect of cyclophosphamide on CD3 T cells in the thymus (Rat)
2.1 POJA-L935
Title: The effect of cyclophosphamide on CD3 T cells in the thymus (rat)
Description: Stain: Immunoperoxidase DAB.
A single injection with cyclophosphamide (CP, 70 mg/ml) induces a transient cortical involution after 4 days, i.e. the dark-blue stained cortex and the lightly stained medulla in normal thymus (A1, A2) turn to darkly stained medulla and a lightly stained cortex (B1, B2) four days after a single injection with CP. The thymus recovers to normal about 10 days later.
(C): Shows a negative Immunoperoxidase control of a normal thymus.
The anti-CD3 monoclonal antibody shows low staining in the normal cortex (A1-2), but medullar thymocytes are CD3+ (A1-1 and A2-1). CD3 expression increases on all T cells upon maturation of the cells they migrate towards the medulla.
Figure B1 and B2 show that, after CP) treatment the strong CD3+ expression in the medulla (B1-1, B2-1) remains unaltered since these non-mitotic cells are not affected by CP. CD3 remains low in the cortex (B2-2).
After ten days (C) the cortex and medulla pattern have repopulated to normal levels as shown in A.
Keywords/Mesh: lymphatic organs, thymus, CD3, cyclophosphamide, rat, toxicologie, immunosuppression, histology, POJA collection
Title: The effect of cyclophosphamide on CD3 T cells in the thymus (rat)
Description: Stain: Immunoperoxidase DAB.
A single injection with cyclophosphamide (CP, 70 mg/ml) induces a transient cortical involution after 4 days, i.e. the dark-blue stained cortex and the lightly stained medulla in normal thymus (A1, A2) turn to darkly stained medulla and a lightly stained cortex (B1, B2) four days after a single injection with CP. The thymus recovers to normal about 10 days later.
(C): Shows a negative Immunoperoxidase control of a normal thymus.
The anti-CD3 monoclonal antibody shows low staining in the normal cortex (A1-2), but medullar thymocytes are CD3+ (A1-1 and A2-1). CD3 expression increases on all T cells upon maturation of the cells they migrate towards the medulla.
Figure B1 and B2 show that, after CP) treatment the strong CD3+ expression in the medulla (B1-1, B2-1) remains unaltered since these non-mitotic cells are not affected by CP. CD3 remains low in the cortex (B2-2).
After ten days (C) the cortex and medulla pattern have repopulated to normal levels as shown in A.
Keywords/Mesh: lymphatic organs, thymus, CD3, cyclophosphamide, rat, toxicologie, immunosuppression, histology, POJA collection